Written by C. Michael Stanley, PhD., Senior Applications Scientist

The use of lasers as the illumination source for fluorescence applications requires special consideration for the optical path. This special attention centers on the coherent nature of the light, the small beam diameter, the polarization, and the power. There are also circumstances where cone angle and scatter within the system play significantly into the design of the optics. While the entire beampath should be considered carefully, this brief note will emphasize the filters and dichroic mirrors used in the beam path.