Answers to the following questions are extremely helpful when discussing the appropriate filters or filter sets for your use.

Which fluorochrome(s) is (are) being used?

What is the wavelength range of excitation and emission for those fluorochromes with which we are not familiar?

What is the make and model of the microscope or instrument being used?

What is the size of the excitation and/or emission filters if a filter wheel is being used in either the excitation or emission path?

Does the filter need to be image quality or not?

This is particularly important for absorption-type emission filters.

What is the maximum thickness possible in the filter mount to be used?

What type of detector is being used e.g. PMT, CCD, film)?

What are the specific reflection and transmission requirements for the dichroic beamsplitter?

Dichroic beamsplitters are designed with various spectral profiles.

No dichroic beamsplitter reflects all wavelengths short of the cut-on wavelengths, nor can it transmit all wavelengths beyond that point.

Being as specific as possible will increase your chances of getting the beamsplitter that you require and will diminish the possibility of paying too much for a beamsplitter that is more than you require.

It is best if we know the excitation and emission filters being used.

This information is particularly important when ordering a dichroic beamsplitter in the absence of any other filters.

Call 1-800-824-7662 or 802-428-2500

Fax 802-428-2525

Email orders [at] chroma [dot] com